Was primarily located within the cytoplasm on the cells, which was observed in 97.eight (263/269) of colon cancer tissues. Among 269 patients, the mean IHC score of Nav1.five expression was drastically greater in tumor tissues than in tumor-adjacent typical tissues (5.1 three.five vs. three.five 2.7, P 0.001; Fig. two). Based on the outcomes from X-tile computer software,Peng et al. Chin J Cancer (2017) 36:Page 4 ofTable 1 Associations of clinicopathologic characteristics with Nav1.five expression in 269 sufferers with colon cancerCharacteristics Clinical parameter Gender Male Female Age (years) 60 60 136 (50.6) 133 (49.4) 98 (36.four) 171 (63.six) 154 (62.6) 92 (37.four) 180 (81.1) 42 (18.9) 228 (84.8) 41 (15.two) 21 (15.four) 17 (12.eight) 12 (12.two) 26 (15.two) 29 (18.eight) six (six.five) 25 (13.9) 6 (14.three) 31 (81.six) 7 (18.4) 115 (84.6) 116 (87.2) 0.503 86 (87.eight) 145 (84.eight) 0.011 125 (81.2) 86 (93.five) 0.947 155 (86.1) 36 (85.7) 0.556 197 (85.three) 34 (14.7) 0.863 138 (51.3) 131 (48.7) 248 (92.2) 21 (7.eight) 17 (six.3) 50 (18.six) 108 (40.1) 94 (34.9) 195 (72.five) 74 (27.five) 61 (22.7) 134 (49.8) 74 (27.5) 51 (19.eight) 206 (80.two) 19 (13.8) 19 (14.5) 35 (14.1) three (14.3) 8 (47.1) 4 (8.0) 9 (8.three) 17 (18.1) 26 (13.3) 12 (16.two) 11 (18.0) 15 (11.two) 12 (16.two) 12 (23.5) 23 (11.two) 119 (86.2) 112 (85.5) 0.983 213 (85.9) 18 (85.7) 0.385 9 (52.9) 46 (92.0) 99 (91.7) 77 (81.9) 0.545 169 (86.7) 62 (83.8) 0.836 50 (82.0) 119 (88.8) 62 (83.8) 0.024 39 (76.five) 183 (88.8) 0.532 145 (53.9) 124 (46.1) 18 (12.4) 20 (16.1) 127 (87.6) 104 (83.9) 0.384 Total individuals Nav1.5 low expression Nav1.five higher expression P valueTumor localization Right-sided colon Left-sided colon Preoperative serum CEA (ng/mL)a five five Preoperative serum CA199 (U/mL)b 35 35 Adjuvant chemotherapy Yes No Pathologic function Tumor size (cm) five five Tumor differentiation Well and moderate Poor T category T1 T2 T3 T4 N category N0 N1-2 TNM stage I II III ER- statusc Low expression Higher expressionAll information are presented as variety of patients followed by percentage inside the parentheses ER- estrogen receptor-, TNM tumor-node-metastasis, CEA carcinoembryonic antigen, CA199 cancer antigen (CA)a b cThe data of preoperative CEA level were accessible in 246 individuals The information of preoperative CA199 level had been offered in 222 individuals ER- status was evaluated in 257 patientsPeng et al. Chin J Cancer (2017) 36:Page five ofFig. 1 Representative immunohistochemical (IHC) staining of voltage-gated sodium channel 1.5 (Nav1.five) protein in tissue microarray (TMA). a1, a2 colon adenocarcinoma with high Nav1.5 expression, with strong intensity and 100 malignant cells staining within the plasma membrane; b1, b2 colon adenocarcinoma with moderate Nav1.2439223-60-4 In stock five expression, with moderate intensity and 75 malignant cells staining inside the cell membrane; c1, c2 colon adenocarcinoma with low Nav1.Price of 2-Bromo-1-cyclohexylethan-1-one five expression, with week intensity and 45 malignant cells staining within the cell membrane; d1, d2 higher Nav1.PMID:23746961 five expression in tumor-adjacent standard tissue, with strong intensity and one hundred epithelial cells staining inside the plasma membrane; e1, e2 moderate Nav1.5 expression in tumor-adjacent normal tissue, with moderate intensity and 80 epithelial cells staining inside the cell membrane; f1, f2 negative Nav1.5 expression in tumor-adjacent standard tissuepathologic TNM stage, acceptance of adjuvant chemotherapy, and ER- expression. The cut-off worth of IHC score in ER- expression was 8. As shown in Table 1, high Nav1.five expression was related with higher preoperative serum CEA level (CEA five ng/mL, P = 0.011). As presented in Fig. three,.